Core facility : Cellular Physiology

Please contact physio.cell.cr.chum@ssss.gouv.qc.ca or visit CRCHUM core facilities web pages for more information.

Services

Metabolism-related determinations

Immunoassay & biochemical determinations
Quantification of hormones, cytokines and peptides (insulin, c-peptide, glucagon, GLP-1, TNFα, leptin, IL-6, etc) in plasma/serum and incubation media via ELISA and AlphaLISA.

Measurement of beta & alpha cell masses by immunohistochemistry
Quantification of beta and alpha cell masses, ß cell / α cell ratio, cell proliferation (Ki-67) and apoptosis (TUNEL) by immunohistochemistry as well as morphometry measurements using an Aperio slide scanner.

Seahorse XF24-3 flux analyzer.The Seahorse XF24-3 instrument analyzes mitochondrial respiration and glycolysis in live cells. More specifically it measures oxygen consumption, CO2 production and extracellular acidification using special detectors (label-free assays).

Operetta high content imaging system
Measurement of various cellular activities by high content fluorescence imaging using the Operetta imager. The Operetta allows the quantification of cell activities such as apoptosis, proliferation, migration, intracellular protein translocation, mitochondrial mass & activities in 96/384 well plate format, in confocal or non-confocal mode.

Equipements

Equipment	Manufacturer	Model
Operetta cellular imager	PerkinElmer	Operetta
Extracellular Flux Analyser	SeaHorse BioScience	XF24-3
EnVision multilabel plate reader	PerkinElmer	2104
Nikon slide scanner	Nikon	LS 9000ED
High definition Aperio slide scanner	Aperio	ScanScope CS
Image analysis software Columbus	PerkinElmer	Columbus
Calorimeter	Parr	6200

Fees

Rental cost for the Aperio slide scanner, Operetta cell imager and SeaHorse analyzer (hourly price)

	without technical support	with technical support
Internal user	10$	45$
Academic user	15$	50$
Company	35$	70$

Annual fee for the use of the Columbus image analysis software

	Annual fee
Internal user	100$
Academic user	N/A
Company	N/A

Immunoassays & Biochemical determinations

*Many other analytes available, contact core personal

* Determinations in tissues are also available.
	Insulin* (rat-mouse-human) ELISA	Insulin * (rat-mouse-human) AlphaLISA	C-peptide* (rat-mouse-human) AlphaLISA	Glucagon (rat-mouse-human) AlphaLISA	TNF-α (rat-mouse) AlphaLISA	Leptin (souris) AlphaLISA	Triglyceride* (plasma)	Free fatty acid* (plasma)	Lactate (plasma)
Internal user	2,25$	1,40$	3,40$	3,40$	2,15$	2,15$	2,50$	1,50$	1,85$
Academic user	2,40$	1,50$	3,50$	3,50$	2,25$	2,25$	2,80$	1,75$	2,20$
Company	3,12$	1,95$	4,60$	4,60$	2,95$	2,95$	3,75$	2,50$	2,95$

Cell mass & morphometry measurements (Price per slide*)

*Do not include scanning on the Aperio slide scanner and image analysis ($35/hour)

Under development : TUNEL assay for Langerhans islets
	Beta-cell mass determination (insulin detection)	Beta-cell mass & proliferation deteminations (insulin & Ki-67 detection)	Beta- & alpha- cell masse determinations (insulin & glucagon detection)	Endocrine cell mass (insulin & chromogranin detection)
Internal user	19$	33$	35$	35$
Academic user	26$	40$	42$	42$
Company	35$	50$	53$	53$

The prices are subject to change without notice.

For more details, please contact :
Erik Joly, PhD
Senior Scientist & Associate Director. Cores & Development
erik.joly@crchum.qc.ca
514-890-8000 x 23608
http://crchum.chumontreal.qc.ca/plateformes-et-servicesspectrometrie-de-masse/metabolomique-et-physiologie-cellulaire

This post is also available in: French